Erratum to “Dietary restriction delays aging, but not neuronal dysfunction, in Drosophila models of Alzheimer's disease.” [Neurobiol. Aging 32 (2011) 1977–1989]

Fig. 4. Analysis of fully fed vs DR food effects on tau levels and phosphorylation in flies over-expressing WT human tau. (A) Tau expression and phosphorylation levels were measured by western blotting in control flies (welav/+, w;UAS-4Rtau/+), and at the indicated time points in welav/+;UAS-4Rtau/+flies treated on1.0 vs 2.0 Y medium. Primary antibodies were as follows: Anti-tau (total tau; Dako, UK), PHF-1 (phospho-Ser396/404 tau), AT8 (phospho-Ser199/202 tau), pS422(phospho-Ser422 tau) and anti-actin. (B) Phospho-tau levels, in welav/+;UAS-4R tau/+flies, were normalised to total tau protein for each sample, and are expressed as average relative intensities SEM. Dietary manipulation did not alter the level or pattern of tau phosphorylation across age at Ser396/404 (P 1⁄4 0.412), Ser199/202 (P 1⁄4 0.838) or Ser422 (P 1⁄4 0.677) epitopes (two-way ANOVA).